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Curriculum Vitae

EDUCATION:

Postdoctoral Research

James Graham Brown Cancer Center, University of Louisville, Louisville, KY, 2006-2008

Focus:  Structure Based Drug Design

Advisor:  Dr. John O. Trent

Doctor of Philosophy, Chemistry (Biochemistry) 

University of Kentucky, Lexington, KY, 2007

Dissertation: Biosynthesis, structure, and function of yersiniabactin, a virulence factor of Yersinia pestis.

Advisor: Dr. Edward DeMoll

Master Of Education (Instructional Design and Development)

University of Georgia, Athens, GA, 2023

Advisor: Dr. Jill E. Stefaniak

Master of Business Administration

Oglethorpe University, Atlanta, GA, 2002

Thesis:  A Customer-Driven Approach to Strategic Decision Making

Advisor:  Dr. James Turner

 

Graduate Certificate: eLearning Design

University of Georgia, Athens, GA, 2023

Advisor: Dr. Jill E. Stefaniak

 

Bachelor of Science, Chemistry

University of Louisville, Louisville, KY, 1999

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EXPERIENCE:

Associate Professor: Department of Chemistry and Biochemistry

University of North Georgia, Dahlonega, GA 2014 – present

  • General Chemistry (CHEM 1211, 1212 with the associated labs.)

  • Survey of General, Organic, and Biochemistry (CHEM 1151, 1152 with associated labs.)

  • Survey of Biochemistry (CHEM 3100)

  • Medicinal Chemistry (CHEM 4824)

  • Biochemistry (CHEM 4841K and 4842K with associated labs.)

  • Plant Biochemistry (CHEM 4926 – Special Topics in Chemistry – Plant Biochemistry)

  • Research (CHEM 490X – Undergraduate Research)

Accomplishments:

  • I reduced the drop rate/fail rate considerably in my general chemistry and introduction to general, organic, and biochemistry.  My current DWF rate is approximately 10%.  This includes all grades lower than a “C” and the students who “withdraw passing”. 

  • I developed the CHEM 4841K/4842K courses with labs and taught them for the first time on the Gainesville campus of UNG.This included developing the accompanying labs for the class and writing a laboratory manual.

  • I authored/co-authored the online templates for CHEM 1152, 1152L, 1152K, 3100, and 4824.

  • I was the first instructor to teach CHEM 4824 (Medicinal Chemistry) online.

  • I introduced, created, and taught Plant Biochemistry (as CHEM 4926 – Special Topics in Biochemistry – Plant Biochemistry) in a collaborative effort with colleagues in the Biology Department.

  • I acquired several instruments for teaching and research at little or no cost to the University or to my department.This equipment includes 3 GC/MS, 3 UV-vis instruments, a LC/MS, a scanning electron microscope equipped with a focused ion beam (an FEI DB235 instrument), basic supplies for the biochemistry lab, a class II biosafety hood, and other materials to be used for my research, for classes, and to support the research efforts of my colleagues.

  • I have been awarded three Presidential Awards for Research.These are competitive awards for internal research funding.

  • I have also excelled in service to my profession, the community, and to the University of North Georgia.I have served on the faculty senate and been voted to the position of Vice-Chair. I have served on over 18 university, college, and departmental committees.I have also served as the faculty advisor to the Chemistry Club on this campus from 2014 until 2019.Additionally, I serve as academic advisor to over 12 students per year.I have been participated in several local STEM outreach events.In 2019 I chaired the local arrangements committee and hosted the annual meeting of the Georgia Academy of Science on the Gainesville Campus of UNG.

  • Instrument Manager for a Waters Arc HPLC equipped with a 2998 photodiode array detector and a Aquity QDa detector.

Adjunct Professor, Department of Bioscience, Division of Life Sciences and Public Safety

Athens Technical College, Athens, Georgia

  • General Chemistry (CHEM 1211) Spring 2018, Fall 2018, Spring 2019, Summer 2019, Fall 2019

  • Survey of General, Organic, and Biochemistry (CHEM 1151 with associated lab) Fall 2020

Research Manager: James Graham Brown Cancer Center, Louisville, KY 2006- 2014

My duties included management of the day to day “wet” laboratory operations of a well-developed in silico drug discovery/translational research effort, training and lab support of graduate students and post docs, trouble-shooting research projects, and maintaining supplies and documentation for the lab.  My responsibilities also included:  

  • Molecular biology for development and maintenance of strains for the production of protein and DNA samples.This includes routine lab methods such as PCR, RT-PCR, site directed mutagenesis, DNA/RNA isolation and purification, and gel electrophoresis.I have experience with a variety of protein expression systems but have focused primarily on and mammalian expression.

  • Purification of protein, DNA, and small molecule samples for biophysical analysis and NMR/crystal structural studies.This includes routine application of purification techniques such as HPLC (most often using size exclusion, ion exchange, and reverse phase chromatography), FPLC (GE Healthcare), affinity chromatography, gel electrophoresis, western blot, and LC/MS.

  • Biophysical characterization of protein, DNA, and protein/DNA/drug interactions using methods such as HPLC, ITC, DSC, UV-vis, CD, and LC/MS.This includes development and troubleshooting of various drug/target assays such as UV thermal shift assay and EMSA.

  • Routine mammalian cell culture propagation for various applications such as cell based assays for drug discovery such as MTT and ApoTox Glo (Promega).This included maintenance and propagation of 50+ mammalian cell lines.

  • Instrument manager for an AKTA Purifier FPLC and two Waters HPLC systems consisting of a 600 pump/2707 autosampler/2998 PDA detector and an Alliance e2695/2998 PDA detector.Each system is equipped with a Fraction collector and runs Empower software.I was often called upon to isolate or quantify protein, DNA, and small molecule samples for various research efforts.

  • Co-manager for the Agilent 6224 TOF LC/MS equipped with the Agilent 1260 UPLC system and MassHunter software.This includes LC/MS analysis of protein, peptide, and DNA samples.I developed and implemented the methods and SOPs for sample preparation and LC/MS analysis of protein and DNA samples for our lab.

SELECTED PUBLICATIONS:

Book Chapters and Invited Papers

1. Tashia Caughran, Greta Giles, and Clarke Miller, Biochemistry Case Studies, Affordable Learning Georgia, November 2020, https://alg.manifoldapp.org/projects/biochemistry-case-studies

 

2. M. Clarke Miller, Ion Exchange | Isolation of Biopolymers, in Encyclopedia of Analytical Science (Third Edition), P. Worsfold, et al., Editors. 2019, Academic Press: Oxford. p. 190-194.

 

3.  M. Clarke Miller, Carl J. Ohrenburg, Ashani Kuttan, and John O. Trent, Separation of Quadruplex Polymorphism in DNA Sequences by Reversed-Phase Chromatography, Current Protocols in Nucleic Acid Chemistry, 2015, 61:17.7.1-17.7.18.

 

4.  M. Clarke Miller and Edward DeMoll, Extraction, Purification, and Identification of Yersiniabactin, the siderophore of Yersinia pestis, Current Protocols in Microbiology, 2011, 23:5B.3.1-5B.3.21

 

5.   M. Clarke Miller and John O. Trent, Size Exclusion Chromatography of G-Quadruplexes, Current Protocols in Nucleic Acids Chemistry, 2011, 45:17.3.1-17.3.18

Peer Reviewed Publications

1.  Kieu Oanh Nguyen, Sayma Al-Rashid, Tom Diggs, Clarke Miller, and Evan Lampert, Trichoplusia ni Hübner (Lepidoptera: Noctuidae) selective sequestration of host plant carotenoids, Environmental Entomology, Volume 48, Issue 3, June 2019, Pages 540–545, https://doi.org/10.1093/ee/nvz029.

2. Alexander Brobov, Olga Kirillina, Marina Fosso Yatchang, Jacqueline Fetherston, M. Clarke Miller, Joe Burlison, Tia VanCleave, William Arnold, Matthew Lawrenz, Sylvie Garneau-Tsodikova, and Robert Perry, Zinc transporters YbtX and ZnuABC are required for the virulence of Yersinia pestis in bubonic and pneumonic plague in mice, Metallomics. 2017 Jun 21;9(6):757-772. doi: 10.1039/c7mt00126f.

3. Subhash Gaddipati, Qingxian Lu, Ramesh Babu Kasetti, M. Clarke Miller, Qingjun Lu, John O. Trent, Henry J. Kaplan, and Qiutang Li, IKK2 Inhibition Using TPCA-1-Loaded PLGA Microparticles Attenuates Laser-Induced Choroidal Neovascularization and Macrophage Recruitment. (2015) PLoS One Mar 24; 10(3):e0121185. doi: 10.1371/journal.pone.0121185.

4. England C.G.*, Miller M.C.*, Kuttan A., Trent J.O., Frieboes H.B., Release kinetics of paclitaxel and cisplatin from two- and three-layered gold nanoparticles., (2015) European Journal of Pharmaceutics and Biopharmaceutics, doi: 10.1016/j.ejpb.2015.02.017. (*Co-First Author)

5.  Alexander G. Bobrov, Olga Kirillina, Jaqueline D. Fetherston, M. Clarke Miller, Joseph A. Burlison, and Robert D. Perry, The Yersinia pestis Siderophore, Yersiniabactin, and the ZnuABC system both contribute to Zinc acquisition and the development of lethal septicemic plague in mice., Molecular Microbiology (2014) Aug;93(4):759-75.

6.  Miller, M.C., Le, H.T., Dean, W.L., Holt, P.A., Chaires, J.B., and Trent, J.O., Polymorphism and resolution of promoter quadruplex-forming sequences., Organic and Biomolecular Chemistry 9 (2011) 7633-7637 (Selected as an OBC “HOT” Article)   

7.  Miller, M.C., Buscaglia, R., Chaires, J.B., Lane, A.N., and Trent, J.O., Hydration is a major determinant of the G-quadruplex stability and conformation of the human telomere 3’ sequence d(A(G3TTA)3G3). Journal of the American Chemical Society 132 (2010) 17105 – 17107

8.  Miller, M.C., Fetherston, J.D., Baker, W., DeMoll, E., and Perry, R.D., Reduced Synthesis of the Ybt siderophore or production of aberrant Ybt-like molecules activate transcription of yersiniabactin genes in Yersinia pestis, Microbiology 56 (2010) 2226-2238

9.  Dailey, M.M.*, Miller, M.C.*, Lane, A.N., Bates, P.J., and Trent, J.O., Resolution and characterization of the structural polymorphism of a single quadruplex forming sequence, Nucleic Acids Research 38 (2010) 4877- 4888 (*Co-First Author, Selected as a NAR Featured Article)

10.  Arumugam, S., Miller, M.C., Maliekal, J., Trent, J.O., and Lane, A.N., Solution structure of the RBD1,2 domains from human nucleolin, Journal of Biomolecular NMR 47 (2010) 79-83

11.  Miller, M.C., Parkin S., Fetherston, J.D., Perry, R.D., and DeMoll, E., Crystal structure of ferric-yersiniabactin, a virulence factor of Yersinia pestis, Inorganic Biochemistry, 100 (2006) 1495-1500

12.   Babb, K., Bykowski, T., Riley, S.P., Miller, M.C., DeMoll, E., and Stevenson, B., Borrelia burgdorferi EbfC, a Novel, Chromosomally Encoded Protein, Binds Specific DNA Sequences Adjacent to erp Loci on the Spirochete’s Resident cp32 Prophages, Journal of Bacteriology, 188 (2006) 4331-4339

Case Studies

1.  Cheryl L. Miller and M. Clarke Miller, Red Blood Cell Alloimmunization, Biochemistry Case Studies, Affordable Learning Georgia, November 2020, https://alg.manifoldapp.org/projects/biochemistry-case-studies

2.  Cheryl L. Miller and M. Clarke Miller, Understanding Solutions in Nursing, Biochemistry Case Studies, Affordable Learning Georgia, November 2020, https://alg.manifoldapp.org/projects/biochemistry-case-studies

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